麻豆淫院

Several studies have shown that a lack of nitrogen in soils adversely affects crop yields. The modern use of nitrogen fertilizers has improved yields to meet expanding global food demand, but in some cases up to 50% of the nitrogen in fertilizers reaches surrounding water bodies in the form of nitrate, causing pollution. As the use of nitrogen fertilizers is rapidly increasing worldwide each year, there is a fundamental need to understand how plants absorb nitrate, and how this absorption can be improved in crops.

In Arabidopsis plants, nitrogen starvation triggers expression of the nitrate transporter gene known as NRT2.4, which allows the plants to absorb trace amounts of nitrate for survival. Now, Takatoshi Kiba and colleagues at the RIKEN Plant Science Center in Yokohama, together with scientists from France and the UK, have gained insight into how NRT2.4 works to benefit nitrogen-starved plants.

鈥淎lthough nitrogen is one of the most important nutrients for plant growth and productivity, how plants sense and respond to levels of nitrogen in soils is not well understood,鈥� explains Kiba. 鈥淭his is why we started the study focusing on the NRT2.4 gene.鈥�

Using green fluorescent proteins and reporter enzymes to help pinpoint the location and presence of the gene, Kiba and his team found a pattern of NRT2.4 expression in the roots and shoots of Arabidopsis seedlings (Fig. 1).

The researchers worked with 10-day-old Arabidopsis seedlings, each weighing around 1 milligram, and measured the tiny amounts of nitrate absorbed by the plants. 鈥淒etection of nitrate influx at very low concentration was the main challenge of this work,鈥� explains Kiba. 鈥淭o obtain valid data, we had to improve the precision of the assay and measurement methods.鈥� They prepared their samples very carefully in assays before measuring nitrate levels with high-performance liquid chromatography and an automated N/C analyzer-mass spectrometer.

The improved precision paid off, because the team鈥檚 results revealed that the NRT2.4 gene is crucial in increasing nitrate absorption by Arabidopsis plants at very low concentrations. However, not all plants are equal. 鈥淥ur preliminary investigation suggests that some domesticated crop plants do not have any mechanism equivalent to NRT2.4 in Arabidopsis,鈥� Kiba explains. 鈥淚t is possible that domesticated plants have lost such a mechanism because it is not necessary in a fertilized environment.鈥�

Introducing the NRT2.4 gene to crops may improve nitrogen uptake efficiency in the future. Kiba notes that 鈥渢his could eventually enable us to reduce nitrogen fertilizer use and to conduct sustainable agriculture easily.鈥�