麻豆淫院

Cell culture is a fundamental technology used in several fields, from basic research in life sciences to medical, energy, and material industries. The culture medium (a solution comprising various nutrients) used to grow cells determines the culture results. Thus, selecting a medium appropriate for cell culture is essential.

Recently, much focus has been placed on developing cell culture media with less effort and without being bound by conventional empirical rules, for which machine learning is used. Nevertheless, only a few study cases have focused on this, and further research is required.

In a new study in the Biochemical Engineering Journal, researchers at the University of Tsukuba investigated the methods for optimizing cell culture media using machine learning. By creating four different machine learning models, they successfully developed culture media that could grow higher cell concentrations. Moreover, they observed that data correction of experimental results for model training improved the performance of machine learning.

Researchers cultured cells derived from human cervical cancer in a medium containing 31 ingredients at various concentrations. The medium compositions and cultured cell concentrations were obtained as training data and subjected to four machine learning models. By applying active learning (repeated machine learning and experimental verification), researchers developed a culture medium that could grow higher cell concentrations than the commercially available medium.

Results revealed that the relative value correction of the training data was crucial for effective machine learning. Moreover, additional gene expression analysis was performed to investigate how the optimized culture medium changed the biological pathways in the cell.

This research provides insights into the development of culture media using artificial intelligence and the know-how for its practical application. This application will benefit cell culture technology and contribute to industry and academic research.