麻豆淫院

Researchers from Aarhus University and the Italian Institute of Technology have discovered how certain proteins can attach to special structures in RNA, called G-quadruplexes. Additionally, they have developed computational tools capable of predicting these protein-RNA interactions. The newfound ability to predict these interactions can help future work in understanding molecular pathways in the cell and pave the way for developing drugs targeting these RNA G-quadruplex binding proteins, that are found to be involved in disease such as cancer.

Proteins binding to RNA are important in many processes in the cell and can mediate a range of biological functions. A specialized structure in both DNA and RNA, the G-quadruplex, consists of regulatory elements involved in gene expression in both DNA and RNA.

In a new study, the researchers used theoretical predictions and molecular biology experiments to show that many chromatin-binding proteins bind to RNA G-quadruplexes. With this information, they can classify proteins based on their potential to bind RNA G-quadruplexes.

The study used a combination of experimental identification of RNA G-quadruplex-binding proteins and computational methods to build a prediction tool that identifies the probability that a protein binds to RNA G-quadruplexes. The findings showed that predicted proteins show a high degree of protein disorder and hydrophilicity, suggesting an involvement in both transcription and phase separation into membrane-less organelles.

Ulf 脴rom's group has previously shown that RNA-DNA dual binding proteins are likely to have an involvement in the DNA damage response, linking DNA and RNA binding properties to a number of proteins. In the study, the researchers expanded the knowledge of RNA-binding proteins to identify RNA G-quadruplex binding proteins.

The researchers have also developed a to assess RNA G-quadruplex-binding potential of proteins.

With these new results, the researchers identify properties of protein-RNA interactions, and provide means to identify G-quadruplex binding properties that can potentially be targeted therapeutically in disease.

The findings have been in Nature Communications.